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Applications
Long Term Potentiation
This experiment can be performed on an acute hippocampal slice on a Multi-Electrode Array (MEA) with 64 channels with the aim to analyze long-term potentiation (LTP) following electrical stimulation.
Paired Pulse Facilitation (PPF) or Depression (PPD)
In Paired Pulse Facilitation (PPF) or Depression (PPD) experiments, the stimulus is followed by a second stimulus, typically 20?80 ms later. The magnitude of the population response to the second stimulus is compared to that of the first one. It is higher in case of a PPF, or lower in case of a PPD.
Spike Activity (Organotypic culture)
Organotypic slices of the dendate gyrus (DG) and the entorhinal cortex (EC) taken from a PND 6 rat were co-cultured on MEAs for seven days. Both subregions showed an independent spontaneous spike activity.
MicroERG's and spikes (Retina)
Stimulation with light pulses results in a complex signalling by neurons within the layers of the retina. The retinal ganglion cells transmit retinal information to higher visual centers in the brain via their axons that form the optic nerve. Retinal function can be affected by acute injuries, intoxications, or retinal diseases, either inherited or acquired, resulting in visual impairment or even blindness.
Application Notes
Acute Hippocampal Slices on Perforated MEAs
A downside of acute slice recordings on MEAs in contrast to for example an interface chamber is that recordings are done from the cells at the bottom of the slice. These cells get less oxygen and nutrients from the perfused ACSF solution and therefore are likely to give smaller signals and might eventually die first. Perforated MEAs present a solution to this problem as they allow a perfusion of the tissue from both sides at the same time, thereby optimizing the oxygen supply of the acute slice.
Retina Recordings (Micro Electroretinograms) from Rattus norvegicus
Here we describe the preparation and handling of rat retina for MEA recordings. This is widely used as an assay to monitor drug effects on the electroretinogram (ERG).
Cortical and Hippocampal Cryopreserved Neurons
This application note includes a complete protocol for the cultivation of ready-to-use cryopreserved primary neurons from QBM Cell Science, suggestions for long term cultures and much more.
Primary Neurons from Cortex or Striatum
See here a complete protocol for the isolation and cultivation of primary cortical neurons, suggestions for long term cultures, suggestions for MEA System configurations, and references.
Primary Hippocampal Neurons from Rattus norvegicus
Here we show a complete protocol for the isolation and cultivation of primary neurons, suggestions for MEA System configurations, and references.
Suprachiasmatic Nucleus Neurons & Organotypic Cultures
This application note includes a complete protocol for the isolation and cultivation of suprachiasmatic nucleus (SCN) neurons, suggestions for long-term cultures, suggestions for MEA System configurations, example data, and references.
Acute Hippocampus Slices from Rattus norvegicus
See here a complete protocol for the dissection of rat brain, the preparation of brain hippocampal slices for acute and long term experiments, suggestions for MEA System configurations, and references.
Preparation of Hippocampal Slices for Organotypic Cultures (OTC)
This application note includes a complete protocol for the dissection of rat brain, the preparation of brain hippocampal slices for acute and long term experiments, suggestions for long-term cultures, suggestions for MEA System configurations, and references.
MC_Rack Tutorials: MEA Application Examples
MC_Rack is a very flexible recording and analysis program that can be customized for various applications. Different software configurations can be saved for later use in so-called virtual rack files, in analogy to a hardware rack configuration. Here you will find a detailed description of demo rack configurations for various typical applications.
We offer a wide range of systems and components for extracellular recordings in vitro on various types of preparations.
See on the left a few of the most important examples of neuronal studies with the MEA-System.
Of course we also offer various system configurations for extracellular recordings in vivo.
See more on these pages:
ME-Systems
USB-ME-Systems
See more detailed online MEA-based protocols here:
for primary neuronal cultuers (hippocampus)
and
for acutely prepared hippocampal slices