Cardio2D

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Overview

Cardio2D is an advanced data acquisition software that is complemented by the analysis module Cardio2D+.

The software supports all generations of Multi Channel Systems’ MEA-Systems and Portable-ME-Systems and is available for free (please see the "Downloads" tab above).

Features include:

Real-time mapping of cardiac propagation
Analysis of heart rate
Calculation of the duration of field potential and conduction velocity.

The given features of this software make it a flexible and versatile tool for drug testing and cardiac electrophysiology research. The software can be used together with classical MEAs to record from cardiac slices and cultured cardiomyocytes (primary, ES and iPS derived cardiomyocytes) or with flexible MEAs for epicardial mappings on isolated Langendorff hearts or in vivo organs.

Please click on the description tab above to learn more about the powerful features of the Cardio2D software package.

Description

Technical Features: Supported Hardware

MEA2100-Mini-60/120-Systems, MEA2100-60/120/256-Systems
MEA2100-Systems allow to record up to 240 channels on up to 4 MEAs simultaneously (4x60, 2x120, 1x256). In the case of 6-well MEAs every single well will be analyzed independently and gets its individual data set and map. Cardio2D supports the full 24 bit resolution and up to 40 kHz sampling capacity of the MEA2100-System.

ME2100-System
Tethered in vivo recording system with integrated stimulation.

USB-ME 32 / 64 / 128 / 256 Systems
The USB-ME series of data acquisition systems can be used for in vivo and Langendorff heart recordings in conjunction with filter amplifiers (FA series) and headstage amplifiers (MPA series). These systems allow also to operate up to four standard MEA60 amplifiers.

Portable-ME-Systems
This compact and versatile system integrates filter amplifier and data acquisition in one unit. It links directly to an MPA32 headstage, which connects to a flexible MEA that can be positioned on the surface of the heart for epicardial mapping or in a pocket in cardiac tissue for transmural mapping. The Portable-ME-Systems offer a sampling rate of up to 40 kHz and a resolution of 16 bit.

Analysis Features: Field potential tab

In the field potential tab, the user can obtain an overview of the experiment. This includes a long-term plot, an overview window as well as an overlay display. The overlay display visualizes the time offset of local activations on the various electrodes of the array. The local voltage distribution is shown in a display plotting voltage versus electrode position. The actual propagation can be displayed and exported as a video file.

Analysis Features: Heartbeat tab

The heartbeat tab displays propagation maps including (optional) isochronous lines. It is possible to identify individual propagation pathways and calculate the conduction velocity along such a path. The timestamps for each electrode are determined either as minimum, maximum or time of maximal slope. Moving the mouse cursor over electrodes will show the detection point as a red bar in the central display. In the top display, there is a long-term plot, that allows jumping from heartbeat to heartbeat.

Analysis Features: Heart Beat Averager

As the determination of specific fiducial points on a raw signal might be challenging, Cardio2D offers the option of averaging signals to improve signal-to-noise ratio. In the Averager, the user can specify the duration of the averages and the trigger for the beginning of each average. Every window has dedicated export functions as graphic or ASCII format.

An overlay window allows comparing different electrodes easily.

Analysis Features: QT Analyzer

The QT Analyzer measures the duration of the field potential on the averaged traces and plots the result as a dose response curve. All data can be exported for external data management. The user has the option to correct detections – this is essential especially in the situation of drug induced arrhythmia. To allow handling variable durations of field potentials and changing beating rates, the average parameters can be adjusted for every drug concentration.