Request a demo MCS Online Shop
The Roboocyte2 is a fully-automated all-in-one solution for medium throughput screenings of ligand-gated and voltage-gated ion channels, as well as electrogenic transporters based on the well established Xenopus oocyte expression system. All necessary tasks are accomplished by a single robot. The automation of TEVC recording revolutionizes pharmaceutical drug discovery:
The Roboocyte2 was designed not only for those familiar with the Xenopus oocyte expression system, but to also encourage others to step into an exciting and new technology for drug discovery.
The Roboocyte2’s compact and functional design saves space on your work bench. It is compatible with standard lab equipment and can be easily integrated in your working environment. Software controls replace any knobs on the device. The Roboocyte2 is straightforward and easy to operate; handling does not require special skills or equipment.
The recording is performed using disposable standard 96-well plates, which are commercially available from several providers. The oocytes are plated into the wells in a couple of minutes. They quickly settle within the conical wells and adhere to the well bottom after a few hours. The oocytes no longer need to leave the plate; you can easily transfer the oocytes from the incubator to the Roboocyte2 device and back again.
The well plate carrier, powered by linear motors, hovers smoothly and noise-free on a cushion of pressurized air above a magnetic steel plate. It operates at 20 μm resolution. The complete system requires no maintenance other than occasional cleaning of the steel plate.
The vertically moving z-arm, which holds the TEVC probe, is designed for the high demand of speed and precision. The z-arm moves at a resolution of 20 μm; position and speed are computer-controlled. A quick adjustment process guarantees the precise impalement of the oocytes. We recommend using the ready-made measuring heads provided by Multi Channel Systems. If you prefer to produce your own, we strongly recommend blank measuring heads from MCS. You can then fix your own capillaries, perfusion tubing, and silver wire to the mounting support.
The new “ClampAmpC” high performance amplifier is specifically designed and manufactured for the two-electrode voltage clamp method by MCS hardware specialists. Ready-to-use TEVC probes make the handling quick and easy. The Roboocyte2 package includes everything you need to start right away, including a high performance computer with monitor, TEVC probes, tubing, the Roboflow liquid handler, and accessories.
Oocyte impalement, compound application, TEVC recording, and online analysis are all performed automatically under computer control. The main characteristic and primary advantage of the Roboocyte2 is its full automation. You design the experiment and define all parameters in advance. At run time, you start the session with a single mouse click. The Roboocyte2 controls the recording for all 96 oocytes in a well plate automatically, even including a wash cycle. Thus the recording can go on 24 hours a day, unsupervised.
The Roboocyte2 software’s easy-to-use graphical user interface makes daily work with the Roboocyte2 quick and easy. Automated software controls replace any knobs on the robot. Customizable responsedependent recording sequences and control routines replace personal supervision.
The Roboocyte2 Java scripting language includes all important commands and functions in a nutshell. This concept makes the Roboocyte2 scripting language sophisticated and powerful, but not redundant or unnecessarily complex. The structure and command names are designed to be very intuitive and user friendly. You do not need any programming skills; a very basic understanding of the general concept is sufficient to harness the full power of scripting.
For quick tests, a convenient manual mode is provided as well. You can manually control all Roboocyte2 actions and parameters that are otherwise automated.
Amongst others, the Roboocyte2 can be used to explore:
Multi Channel Systems has a team of highly-qualified scientists, whose only task is to help you with any issue you might have. No matter whether you want to know whether your biological sample is suitable for MEA recordings or if you have a specific software question: Our support team is here to help you.
We have scientists who earned their PhDs in the fields of biology, biophysics, and biochemistry and specialized in:
Our sales team has years of experience in electrophysiology, too. They can help you in choosing the right system for your specific application, give you advice on the general set-up and useful accessories. They are happy to visit you in your lab anytime to:
Worldwide, we are supported by our distribution partners. We have long-lasting cooperations with all of our partners and they are regularly trained on our equipment. Be sure that they have the expertise to advise you on all MCS-Systems.
Please do not hesitate to contact us. Normally, we reply to you within 24 hours. You can contact us via:
Fully-automated Roboocyte2 system for screening of ligand-gated and voltage-gated ion channels, as well as electrogenic transporters based on the standard Xenopus oocyte expression system. Complete with Roboocyte2 robot, 12-channel perfusion system, data acquisition and analysis software package, computer, and accessories.