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Innovations in Electrophysiology

LTP-Director

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Overview

LTP Director is a specialized acquisition software that is complemented by the analysis module LTP Analyzer. The software supports MEA2100-Systems (MEA2100-Mini excluded), MEA1060-BC ampilifiers with external STG as well as the ME64/128/256 data acquisition systems. Stimulation, recording and electrode selection are controlled from within one software module. In comparison to MC_Rack or Multi Channel Suite, the LTP Director is less versatile, but much easier to use for its main purpose: LTP experiments in acute hippocampal slices.

Features include:

Control of stimulation electrodes, stimulation patterns, recording parameters and experimental design from within one software.
Two independent stimulation pathways.
Automatic detection and on line analysis of EPSPs and population spikes.
Integrated documentation saved together with the data.
Fully automated, pre-defined experiments.
Export of already normalized results as ASCII data.
Generation of report sheets for each experiment.

The given features of this software make it an easy to use tool for standardized LTP experiments, which can be operated by technical personal without problems.

Please click on the description tab above to learn more about the powerful features of the LTP Director Software package.

Description

Experimental Control:

Select stimulation electrodes by mouse click on a slice image. Four electrodes are selected for analysis online, all electrode are recorded. Stimulation parameters for two independent pathways are easily adjusted. Paired pulse paradigms are possible.

Online Documentation:

All relevant experimental parameters can be documented in an online lab book, which will be stored together with the data. At the end of each experiment, an automatic report can be generated, which includes all information from the lab book and an overview of the results.

Signal Detection and Analysis:

Waveforms of EPSPs and population spikes are automatically detected, and all relevant parameters are extracted simultaneously:

EPSP slope and amplitude, area and latency.
Population spike amplitude, area and latency.
Paired Pulse ratio for all analyzed parameters, if applicable

Experiment Setup:

The workflow of the experiment can be designed by selecting the sequence and respective length of different experimental modules. If an automated drug application system is used, the experiment runs fully automated.

Off Line Data Analysis:

The Analysis Tool LTP-Analyzer can be installed on as many computer as needed with no extra cost. A single mouse click shows raw data traces to each data point. Experimental results can be normalized for each channel to the mean of the baseline and exported as ASCII directly.