Foresee IntraCell & MCS MEA Systems

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Overview

IntraCell & MCS MEA Systems

We’ve partnered with Foresee Biosystems to combine groundbreaking technologies that set new standards for intracellular electrophysiology. IntraCell is a laser-based optoporation system that, when used in conjunction with a Multi Channel Systems microelectrode array (MEA) system, allows researchers to capture high-quality intracellular action potentials (APs) from both 2D cardiac monolayers and 3D cardiac organoids.

IntraCell offers new opportunities in acute and especially chronic cardiotoxicology and safety pharmacology studies, enabling repeated recording sessions for 35+ days within the same cells without compromising their viability.

Our solution integrates the IntraCell unit with our latest MCS MEA systems, along with the corresponding software tools designed for precise data analysis. Our PhD experts are available to help you choose the right instrumentation for your unique research needs.

Description

How It Works

The IntraCell system uses an ultra-short-pulsed laser to create transient nanopores in the membranes of cardiac cells cultured on a MEA. This process allows the recording of action potentials (APs) from the cells.

The laser alignment and scanning process is fully automated and guided by the software, and specific MEA layouts and electrodes can be selected for the recordings.

IntraCell’s advanced technology facilitates detailed analysis of cardiac cell behavior, including real-time video recordings and contractility measurements, and is a powerful tool for studying the effects of various compounds on heart cells.

Acute and Long-Term Studies

IntraCell makes both acute or long-term studies possible. Detect intracellular and extracellular potentials within your cell culture for 35+ days.

Measurements from Same Cell Cultures

Perform electrophysiological measurements and optical imaging from the same cell cultures at the same time. This allows for easy observation of beating cells.

Increased Throughput

Increase your throughput compared to traditional methods. With the modular system from MCS you can upscale the system for up to 8 independent MEA experiments at once.

Ideal for Safety Pharmacology and Cardio Toxicity Studies.

The solution is ideal for experiments with primary and stem cell derived cardiac cell cultures and cardiac organoids.

Source: https://link.springer.com/article/10.1007/s00204-022-03422-y

Flexible and Multi-Purpose MEA System

In addition to being used with the IntraCell system, our modular MEA system can also be used anytime as a standalone system for traditional MEA studies on a lab bench or inside an incubator.

Replace, Reduce, Refine (3R)

By utilizing human stem-cell derived cardiomyocyte assays and organoids, researchers can effectively assess the proarrhythmic potential of new drugs without using animal models. The IntraCell solution allows for easy observation of beating cells in vitro and can help labs meet their 3 R’s goals (Replacement, Reduction, Refinement) as well as adhere to CiPA (Comprehensive In Vitro Proarrhythmia Assay) initiative standards.

Benefits

HIGH RESOLUTION: IntraCell technology allows for the measurement of intracellular action potentials simultaneously from several MEA electrodes, up to 256 parallel recordings.
LOW INVASIVENESS: The innovative technique of optoporation, which is used to locally open nanopores in the cell membrane, does not have a significant impact on cell viability over an extended time.
LONG-TERM RECORDINGS: You can study the electrophysiology of the same cells on top of an electrode for over 35 days, analyzing the variations of the action potential over time at different conditions.
REAL-TIME IMAGING: The integrated video microscope enables brightfield imaging of the cells as well as the observation and documentation of the rhythmic contraction of the cardiac myocytes.
EXCITATION-CONTRACTION COUPLING: By measuring simultaneously APs and contractility in the same experiment, you can easily study the excitation-contraction (E-C) coupling of the heart.

Products

Documents

Data sheet:

MCS_MEA2100-Mini-System_Datasheet.pdf

Brochure:

MCS_MEA2100-Mini-System_Flyer.pdf

MEA2100-256_Flyer.pdf

Publications

Iachetta, G., Melle, G., Colistra N., Tantussi F., De Angelis F., Dipalo M. Archives of Toxicology, Volume 97, 2023: Long-term in vitro recording of cardiac action potentials on microelectrode arrays for chronic cardiotoxicity assessments
URL: https://link.springer.com/article/10.1007/s00204-022-03422-y
Schaefer, J., Danker, T., Gebhardt, K., Kraushaar, U. (Indipendent Publication) Jove Journal, Volume 187, 2022: Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology
URL: https://www.jove.com/t/64355/laser-induced-action-potential-like-measurements-cardiomyocytes-on
Melle G., Bruno G., Maccaferri N., Iachetta G., Colistra N., Barbaglia A., Dipalo M., De Angelis F., Frontiers in Bioengineering Biotechnology, 2020: Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms
URL: https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00066/full
Dipalo M., Rastogi S.K., Matino L., Garg R., Bliley J., Iachetta G., Melle G., Shrestha R., Shen S., Santoro F., Feinberg A.W., Barbaglia A., Cohen-Karni T., De Angelis F., Science Advances, Volume 7, 2021: Intracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes
URL: https://www.science.org/doi/10.1126/sciadv.abd5175

www.multichannelsystems.com

Innovations in Electrophysiology

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IntraCell & MCS MEA Systems

IntraCell

MEA2100-Mini-60-System

MEA2100-Mini-120-System

MEA2100-256-System

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Foresee IntraCell & MCS MEA Systems

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IntraCell & MCS MEA Systems

IntraCell

MEA2100-Mini-60-System

MEA2100-Mini-120-System

MEA2100-256-System