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The PatchServer can patch a cell and establish the requested recording configuration fully automatically without any user interference. If desired, the user has the option to online interact with the patch process. The user can fine-tune and supervise the patch process using controls within the PatchServer software with a seamless range of control from “not interfering - automatic” to “full manual control”.
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Another cost saving factor is the possibility to visually identify the cell that is about to be patched, before the recording configuration is established. Thereby, no time and resources are wasted doing experiments on cells that would not be in the later analysis anyway. Furthermore, the small volumes (~30 mm3) of the individual application bays are an advantage, because little volumess of solution are sufficient to perform a complete wash out.
The PatchServer has been tested with:
The PatchServer is worldwide the only automatic patch-clamp system that allows the user to visually identify the targeted cell. After identification, the user has two options:
Having this choice has several advantages for the user. Most importantly, it saves time and resources. The user does not have to perform experiments with cells that do not fit the desired criteria. Furthermore, it is possible to patch specific cells from a solution of acutely dissociated cells, like when they are prepared from specific parts of acute brain slices.
The PatchServer utilizes standard glass electrodes and follows step-by-step the manual patch method to establish the desired recording configurations. As a direct consequence of this approach, the PatchServer produces data quality that is similar to the Gold-standard quality of manual setups. In addition, the PatchServer’s data consistency and production rate is much higher compared to the manual approach of a human experimenter. This higher rate is achieved due to the PatchServer’s applied automatism that will always produce the same high quality recording configurations, because each recording configuration is established individually, with permanent feedback control during the sealing and break-in process.
In addition, throughout the experiment the PatchServer continuously monitors important, quality-defining parameters of the recording configuration and automatically safes those in a specific data file. This enables the user to validate the results during the experiment, but also later offline during the data analysis. This allows for pharmaceutical-screening grade data production and in general eliminates the consideration of data points that would otherwise falsify the end result. Amongst others, the most important monitored parameters are: RecordingPipette resistance (Rpip), access resistance (Ra), cell membrane capacitance (Cm) and holding current (Ih). Other parameters are protocol values like holding potentials and values of the test protocols.
Multi Channel Systems has a team of highly-qualified scientists, whose only task is to help you with any issue you might have. No matter whether you want to know whether your biological sample is suitable for MEA recordings or if you have a specific software question: Our support team is here to help you.
We have scientists who earned their PhDs in the fields of biology, biophysics, and biochemistry and specialized in:
Our sales team has years of experience in electrophysiology, too. They can help you in choosing the right system for your specific application, give you advice on the general set-up and useful accessories. They are happy to visit you in your lab anytime to:
Worldwide, we are supported by our distribution partners. We have long-lasting cooperations with all of our partners and they are regularly trained on our equipment. Be sure that they have the expertise to advise you on all MCS-Systems.
Please do not hesitate to contact us. Normally, we reply to you within 24 hours. You can contact us via:
The PatchServer can patch a cell and establish the requested recording configuration fully automatic without any user interference. If desired, the user has the option to online interact with the patch process. The user can fine-tune and supervise the patch process using controls within the PatchServer software with a seamless range of control from “not interfering - automatic” to “full manual control”.
Due to the 4 pipette holder array, the PatchServer increases the data production rate compared to a manual patch-clamp setups and its pipette based patch approach guarantees low operating costs. Compared to other automatic patch-clamp systems, the PatchServer is the only system that allows the user to identify the cells before they are used for an experiment.
One-channel PatchServer setup for automatic establishment of any patch clamp recording configuration on suspended cells. Includes main unit, platform, catch pipette with amplifier, software, data acquisition computer with preinstalled software, and accessories.
Four-channel PatchServer setup for automatic establishment of any patch clamp recording configuration on suspended cells. Includes main unit, microscope stage, catch pipette with amplifier, software, data acquisition computer with preinstalled software, and accessories.
• HEKA, EPC 10, single patch clamp amplifier
• HEKA, PATCHMASTER software
• HEKA, EPC 10, quadro patch clamp amplifier
• HEKA, PATCHMASTER software
Microscope with camera for PatchServer
• Inverse Microscope & Accessories
- Olympus, inverted microscope, IX73
- Olympus Objectives
- IDS, UI-1550LE
• Supply voltage: 110 / 230 V
Technical specifications as specified in the data sheet unless noted otherwise in the order.
Anti-vibration table and Faraday cage
• Faraday cage
- TMC, Type II Faraday Cage with armrest pads (750x1200 mm, height: 102 cm (40 in), 3 cable holes: 5 cm (2 in) diameter)
• Anti-vibration table
- TMC, 63-500 isolation table + Clean Top (750x1200, M6 on 25 mm)
• Full perimeter enclosure (750x1200x50 mm)
• Ultra-Fast-Application (UFA) Tool
- Optional hardware accessory for PatchServer enabling sub-millisecond solution exchanges based on a theta-capillary mounted on a piezo actuator.
- Includes high-voltage electronics, a piezo-actuator with 60 µm travel, and a set of two theta-capillaries.
Installation and user training in the laboratory for two days
• Installation of hard- and software:
- Setting up the system
- Installation of all necessary software programs
- Functional test of hardware
- Explanation of software and hardware
- Training with cells by arrangement